BioVision Inc Annexin V-PE Apoptosis Kit Plus: Annexin V-PE Apoptosis Detection Kit Plus: Simple One Step Assay to Detect Apoptotic, Necrotic & live cells within 10 min. No need to fix the cells. Detection: Flow Cytometry or fluorescence microscopy.
Manufacturer: BioVision Inc K20325
Annexin V-PE Apoptosis Kit Plus: The Annexin V-PE Apoptosis Detection Kit Plus is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine from the inner face of the plasma membrane to the cell surface. The Annexin V-PE Apoptosis Detection Kit includes annexin V-PE, SYTOX green dye, and binding buffer. SYTOX green dye is impermeable to live cells and apoptotic cells, but stains necrotic cells with intense green fluorescence by binding to cellular nucleic acids. After staining a cell population with annexin V-PE and SYTOX Green dye in the provided binding buffer, apoptotic cells show red fluorescence, dead cells show green fluorescence and live cells show little or no fluorescence. Populations can distinguished by Fluorescence microscopy using FITC and PE filters or by flow cytometry using the FL1 channel (Ex. 488 nm/Em. 530 nm) for SYTOX Green dye and FL2 channel for Annexin V-PE (Ex. 488 nm/Em. 578 nm).
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