4EBP1 (pT69) Mouse anti-Human, PE, Clone: M34-273, BD
Mouse Monoclonal Antibody
Manufacturer: BD Biosciences 560288
The eukaryotic translation initiation factor 4E-Binding Protein hosphorylated heat- and acid-stable protein (PHAS-I or PHAS-1), and it is regulated by insulin. It is a member of the eIF4E-Binding Protein Family, which also includes the proteins 4EBP2 and 4EBP3. 4EBP1 binds with eukaryotic translation Initiation Factor 4E (eIF4E), which prevents its assembly into the eIF4E complex and inhibits cap-dependent translation. When 4EBP1 is phosphorylated, this binding is disrupted, allowing cap-dependent translation to be activated. Phosphorylation of 4EBP1 is required for protein synthesis, and it mediates the regulation of protein translation by stimuli that signal through the phosphoinositide 3 (PI3) kinase pathway. We found that threonines 36 and 45 (T36/T45) are phosphorylated in resting human peripheral blood mononuclear cells. PI3 kinase inhibitors, such as LY294002 down-regulate the phosphorylation level of 4EBP1 (pT36/pT45).
The M34-273 monoclonal antibody recognizes the phosphorylated T69 of activated human 4EBP1.
|4E-BP1. EIF4EBP1, P/OKCL.6, PHAS-I, PHAS-1|
|Phosphorylated Human 4EBP1 (pT69) Peptide|
|Aqueous buffered solution containing BSA and ≤0.09% sodium azide.|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
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