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5-Methylcytosine Sheep anti-Chemical, Polyclonal, Invitrogen™

Sheep Polyclonal Antibody

Manufacturer:  Invitrogen PA130675

Catalog No. PA130675


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Description

Description

PA1-30675 detects 5-Methyl Cytosine from various samples. PA1-30675 has been successfully used in immunohistochemistry (frozen tissue), immunoprecipitation, and Western blot applications. The use of 7mM NaOH in the IP is crutial. The PA1-30675 immunogen is the 5-methyl cytosine molecule conjugated to KLH. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.

Methylation of DNA cytosine bases plays important roles in the regulation of gene transcription. It has been estimated that 60 to 90% of the cytosines in CpG dinucleotides are methylated. An increase in methyl-CpG correlates with transcriptional silencing for the whole chromosome especially in developmentally regulated genes. Methylation is believed to lead to transcriptional silencing by multiple mechanisms including alteration of transcription factor binding to promoters and alteration in chromatin structure.
Specifications

Specifications

5-Methylcytosine
Polyclonal
Unconjugated
Liquid
Sheep
IgG
250 μg
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Primary
Chemical
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen), Immunoprecipitation, Western Blot
3 mg/mL
0.15M PBS with no preservative; pH 7.5
5mc; 5-mC; 5-meC; C5H7NO3; 5-Methyl-cytosine; Methylcytosine
5-methyl cytosine molecule conjugated to KLH.
Purified
RUO
Antibody
Polyclonal
Documents
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