Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

6x-His Tag Rabbit anti-Tag, HRP, Polyclonal, Invitrogen™

Rabbit Polyclonal Antibody

Manufacturer:  Invitrogen PA1983BHRP

Catalog No. PA1983BHRP


Add to cart

Description

Description

PA1-983B-HRP detects His-tagged proteins in Western Blot applications.

The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombit proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombit protein using genetic engineering. By choosing a particular epitope and recombit protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombit proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.
Specifications

Specifications

6x-His Tag
Polyclonal
HRP
Liquid
Rabbit
IgG
50 μL
4° C, do not freeze
Primary
Tag
Western Blot
1 mg/mL
PBS with proprietary stabilizer and 0.07% Kathon
His Tag; Histidine Tag; Histidine Epitope Tag; 6X His eptope tag; 6His tag; 6X His
Synthetic peptide corresponding to residues H H H H H H
Affinity chromatography
RUO
Antibody
Polyclonal
Documents
Provide Content Correction

We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit