6x-His Tag Chicken anti-Tag, Polyclonal, Invitrogen™
Chicken Polyclonal Antibody
Manufacturer: Invitrogen PA19531
PA1-9531 has been successfully used in Western blot, ELISA and immunocytochemistry procedures. By Western blot, PA1-9531 detects a His-Tag fusion protein protein representing His Tag. PA1-9531 immunogen is a His tagged synthetic peptide.The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombit proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombit protein using genetic engineering. By choosing a particular epitope and recombit protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombit proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.
|-20° C, Avoid Freeze/Thaw Cycles|
|ELISA, Immunocytochemistry, Western Blot|
|PBS with 0.02% sodium azide|
|His Tag; Histidine Tag; Histidine Epitope Tag; 6X His eptope tag; 6His tag; 6X His|
|His tagged synthetic peptide.|
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