AAK1 Rabbit anti-Human, Mouse, Rat, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA520616
A suggested positive control is rat spleen tissue lysate. PA5-20616 can be used with blocking peptide PEP-0736.AP2-associated protein kinase 1 (Aak1) is a member of the Ark1/Prk1 subfamily of Ser/Thr protein kinases that are thought to regulate endocytosis by phosphorylating the accessory endocytic components. Aak1 interacts with and phosphorylates the mu2 subunit of the AP-2 complex, which promotes binding of the AP-2 to tyrosine based (Yxxphi) internalization motif-containing receptors and subsequent receptor endocytosis. At least two isoforms of Aak1 are known to exist; the longer isoform contains an extended carboxy-terminus that contains an additional clathrin-binding domain. Overexpression of this long isoform or Aak1 depletion by RNA interference impairs transferrin recycling from the early/sorting endosome, suggesting that Aak1 functions at multiple steps of the endosomal pathway by regulating transferrin internalization and its recycling back to the plasma membrane.
|A 20 amino acid peptide near the amino terminus of the human Aak1.|
|Antigen affinity chromatography|
|Human, Mouse, Rat|
|Immunocytochemistry, Immunofluorescence, Western Blot|
|PBS with 0.02% sodium azide|
|Q2M2I8, Q3UHJ0, P0C1X8|
|AP2-associated protein kinase 1; adaptor-associated kinase 1|
|Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|22848, 269774, 500244|
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