Active Caspase-3 Rabbit, BUV395, Clone: C92-605, BD
Rabbit Monoclonal Antibody
Manufacturer: BD Biosciences 564095
The caspase family of cysteine proteases plays a key role in apoptosis and inflammation. Caspase-3 is a key protease that is activated during the early stages of apoptosis and, like other members of the caspase family, is synthesized as an inactive pro-enzyme that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another protease. The processed forms of caspases consist of large (17-22 kDa) and small (10-12 kDa) subunits which associate to form an active enzyme. Active caspase-3, a marker for cells undergoing apoptosis, consists of a heterodimer of 17 and 12 kDa subunits which is derived from the 32 kDa pro-enzyme. Active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cytoplasm, e.g., D4-GDI and Bcl-2, and in the nucleus (e.g. PARP). This antibody has been reported to specifically recognize the active form of caspase-3 in human and mouse cells. It has not been reported to recognize the pro-enzyme form of caspase-3.
The antibody was conjugated to BD Horizon BUV395 which is part of the BD Horizon Brilliant Ultraviolet family of dyes. This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors. With an Ex Max at 348nm and an Em Max at 395nm, BD Horizon BUV395 can be excited with a 355nm laser and detected with a 379/28 filter.
|CPP32; Yama; Apopain|
|Human Active Caspase-3 Fragment|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
|Brilliant Ultraviolet 395|
|Aqueous buffered solution containing BSA and ≤0.09% sodium azide.|
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