AIF Rabbit anti-Human, Mouse, Rat, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA130677
PA1-30677 detects AIF from human, mouse, rat samples. PA1-30677 has been successfully used in Western blot applications. The PA1-30677 immunogen is: Synthetic peptide - amino acids 517-531 of human AIF. This sequence is identical to those of mouse and rat AIF Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.Apoptosis Inducing Factor (AIF) causes chromatin condensation and DNA fragmentation. AIF was recently identified, and cloned. Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection and apoptosis stimuli. AIF is highly conserved between human and mouse and widely expressed. Mutations in the AIF gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants of AIF and a related pseudogene has been identified on chromosome 10.
|Synthetic peptide corresponding to amino acids 517-531 of human AIF. This sequence is identical to those of mouse and rat AIF|
|Antigen affinity chromatography|
|Human, Mouse, Rat|
|Immunohistochemistry (Paraffin), Western Blot|
|PBS with 0.02% sodium azide; pH 7.4|
|O95831, Q9Z0X1, Q9JM53|
|RP3-438D16.2, AIF, MGC111425, PDCD8|
|Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|26926, 83533, 9131|
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