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AKT1/AKT2 Rabbit anti-Human, Polyclonal, Invitrogen™

Rabbit Polyclonal Antibody

Manufacturer:  Invitrogen PA137053

Catalog No. 01-673-037


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Description

Description

This antibody is predicted to react with bovine, chicken, canine, amphibian, mouse, porcine and rat based on sequence homology. Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is lung carcinoma.

AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at  ft.Ser-50 ft. negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling. Phosphorylation of TBC1D4 triggers the binding of this effector to inhibitory 14-3-3 proteins, which is required for insulin-stimulated glucose transport. AKT regulates also the storage of glucose in the form of glycogen by phosphorylating GSK3A at  ft.Ser-21 ft. and GSK3B at  ft.Ser-9 ft., resulting in inhibition of its kinase activity.
Specifications

Specifications

AKT1/AKT2
Polyclonal
Unconjugated
AKT2
Liquid
AKT1, AKT2
Synthetic peptide from N-terminus of human Akt1
Antigen affinity chromatography
RUO
Antibody
Polyclonal
Human
Immunohistochemistry (Paraffin)
0.75 mg/mL
PBS with 1% BSA and <0.1% sodium azide; pH 7.6
P31749, P31751
RAC-alpha serine/threonine-protein kinase, Protein kinase B, PKB, Protein kinase B alpha, PKB alpha, Proto-oncogene c-Akt, RAC-PK-alpha, AKT1, PKB, RAC, RAC-beta serine/threonine-protein kinase, Protein kinase Akt-2, Protein kinase B beta, PKB beta, RAC-PK-beta
Rabbit
IgG
1 mL
4° C, do not freeze
Primary
207, 208
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