Alpha A Crystallin Rabbit anti-Bovine, Rat, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA1009
PA1-009 detects alpha-A crystallin from bovine and rat samples. This antibody does not detect the unphosphorylated form of the protein. PA1-009 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects an ~20 kDa protein representing alpha-A crystallin from bovine lens samples. Immunohistochemical staining of alpha-A crystallin in rat thymus samples results in staining of epithelial reticular cells. The PA1-009 immunogen is purified alpha-A Crystallin from bovine lens.Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products--alpha-A and alpha-B. There are at least 5 different proteins comprising the beta-crystallins. The gamma-crystallins are monomeric, but there are at least 5 gamma crystallins identified in bovine and rat lens. Alpha-Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintece. Alpha-B crystallin has been linked to Alexander’s disease where it accumulates in brain cells of those afflicted.
|alpha A Crystallin|
|PBS with 30mg/mL BSA and 0.05% sodium azide|
|Purified alpha-A Crystallin from bovine lens.|
|Antigen affinity chromatography|
|Immunohistochemistry (Paraffin), Western Blot|
|-20° C, Avoid Freeze/Thaw Cycles|
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