alpha Internexin Chicken anti-Bovine, Equine, Human, Mouse, Porcine, Rat, Polyclonal, Invitrogen™
Chicken Polyclonal Antibody
Manufacturer: Invitrogen PA110009
In Western blot, human proteins run at ∽68 kDa, while rat and mouse run lower at ∽66 kDa. Minor bands at ∽150 kDa are probably covalent dimers, and bands at ∽50 kDa represent alpha-internexin breakdown products. Stable at 4°C for at least one year. Mix 1:1 with 100% glycerol and store at -20°C for longer term storage.Alpha internexin is a Class IV intermediate filament originally discovered as it copurifies with other neurofilament subunits. On SDS-PAGE gels it runs with an apparent molecular weight of 64 to 66 kDa, with some species variability, although the real molecular weight is about 55kDa; as with the other neurofilament subunits the presence of highly negatively charged sequences results in reduction of SDS-PAGE mobility. a-internexin is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. It is expressed only in neurons and in large amounts early in neuronal development, but is down-regulated in many neurons as development proceeds. Many classes of mature neurons contain a-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons a-internexin is the only neurofilament subunit expressed. Antibodies to a-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition, the very early developmental expression of a-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cells committed to the neuronal lineage. In addition, recent studies show a marked up-regulation of a-internexin during neuronal regeneration.
|PBS with 5mM sodium azide|
|P23565, P46660, Q08DH7, Q16352|
|FLJ18662, FLJ57501, MGC12702, NEF5, NF-66, TXBP-1|
|Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|100059769, 100153400, 226180, 24503, 532236, 9118|
|Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot|
|Purified, recombit rat alpha-internexin, expressed in E. coli.|
|Bovine, Equine, Human, Mouse, Porcine, Rat|
We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.
Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.Ok