alpha Internexin Chicken anti-Bovine, Equine, Human, Mouse, Porcine, Rat, Polyclonal, Invitrogen
Chicken Polyclonal Antibody
Manufacturer: Invitrogen PA110009
In Western blot, human proteins run at ~68 kDa, while rat and mouse run lower at ~66 kDa. Minor bands at ~150 kDa are probably covalent dimers, and bands at ~50 kDa represent alpha-internexin breakdown products. Stable at 4°C for at least one year. Mix 1:1 with 100% glycerol and store at -20°C for longer term storage.Alpha internexin is a Class IV intermediate filament originally discovered as it copurifies with other neurofilament subunits. On SDS-PAGE gels it runs with an apparent molecular weight of 64 to 66 kDa, with some species variability, although the real molecular weight is about 55kDa; as with the other neurofilament subunits the presence of highly negatively charged sequences results in reduction of SDS-PAGE mobility. a-internexin is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. It is expressed only in neurons and in large amounts early in neuronal development, but is down-regulated in many neurons as development proceeds. Many classes of mature neurons contain a-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons a-internexin is the only neurofilament subunit expressed. Antibodies to a-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition, the very early developmental expression of a-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cells committed to the neuronal lineage. In addition, recent studies show a marked up-regulation of a-internexin during neuronal regeneration.
|PBS with 5mM sodium azide|
|P23565, P46660, Q08DH7, Q16352|
|FLJ18662, FLJ57501, MGC12702, NEF5, NF-66, TXBP-1|
|Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|100059769, 100153400, 226180, 24503, 532236, 9118|
|Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot|
|Purified, recombit rat alpha-internexin, expressed in E. coli.|
|Bovine, Equine, Human, Mouse, Porcine, Rat|
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