PAK2 Rabbit anti-Human, Rat, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA137067
This antibody is predicted to react with bovine and mouse based on sequence homology. Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is breast carcinoma.Alpha PAK p68, beta PAK p65 and gamma PAK p62 are three isoforms of serine/threonine kinases. These three isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation.
|PBS with 1% BSA and <0.1% sodium azide; pH 7.4|
|PAK65, PAKgamma, PAK-2, S6/H4 kinase, gamma-PAK, p21 CDKN1A-activated kinase 2, p21-activated kinase 2, p58, serine/threonine-protein kinase PAK 2|
|Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Immunohistochemistry (Paraffin), Western Blot|
|Synthetic peptide corresponding to aa 525-545 of rat alpha PAK|
|Antigen affinity chromatography|
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