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alpha Tubulin Mouse anti-Many, Clone: TU-01, Invitrogen™

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen MA119162

Catalog No. 01-675-932


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Description

Description

This antibody recognizes the defined epitope (aa 65-97) on N-terminal structural domain of alpha-tubulin. HPB-ALL human peripheral blood leukemia cell line is the suggested positive control (incubate for 90 mins). Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.

Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predomitly found in morphologically differentiated neurologic cells.
Specifications

Specifications

alpha Tubulin
Monoclonal
1 mg/mL
PBS with 15mM sodium azide; pH 7.4
P81948, P68366, P68368, Q5XIF6
FLJ30169, H2 ALPHA, Testis specific alpha tubulin, TUBA1, Tubulin alpha
Mouse
IgG1
100 μg
4° C, do not freeze
Primary
22145, 316531, 7277, 777775
Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
TU-01
Unconjugated
TUBA4A
Liquid
TUBA4A
Fraction of tubulin purified from porcine brain by two cycles of polymerization - depolymerization.
selective precipitation
RUO
Antibody
Monoclonal
Many
Documents
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