AMBRA1 Rabbit anti-Human, Mouse, Rat, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA520512
A suggested positive control is rat brain tissue lysate. PA5-20512 can be used with blocking peptide PEP-0632.Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. Beclin-1, a principal regulator of autophagosome formation, is in turn regulated by Ambra1. Ambra1 associates with Beclin-1 through a region near its center as determined by yeast two-hybrid assay. Null mutations in this gene in mice resulted in embryonic lethality with severe neural tube defects associated with autophagy impairment, accumulation of ubiquitinated proteins, unbalanced cell proliferation and excessive apoptotic death. Furthermore, down-regulation of Ambra1 in cultured cells though RNA interference decreased the level of rapamycin- and nutrient starvation-induced autophagy. Multiple isoforms of Ambra1 are known to exist.
|An 18 amino acid peptide from near the amino terminus of human Ambra1.|
|Antigen affinity chromatography|
|Human, Mouse, Rat|
|Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot|
|PBS with 0.02% sodium azide|
|DCAF3, WDR94; DDB1 and CUL4 associated factor 3; WD repeat domain 94; activating molecule in BECN1-regulated autophagy protein 1; activating molecule in beclin-1-regulated autophagy|
|Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|228361, 55626, 59319|
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