Angiopoietin 2 Rabbit anti-Human, Mouse, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA129640
PA1-29640 detects Angiopoietin 2 from human and mouse samples. PA1-29640 has been successfully used in Western blot applications. There is reaction with serum in the cell supernatants, which results in strong background and makes it difficult to see the angiopoietins in cell supernatants. However when precipitated (using soluble Tie2) the signals are very good and strong. The PA1-29640 immunogen is: Synthetic peptide: amino acids 21-40 of Mouse Angiopoietin 2. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.Angiopoietin-1 (Ang-1) and Antiopoietin-2 (Ang2) are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-2 expression occurs at sites of vascular remodeling: dorsal aorta and major aortic branches, ovary, placenta and uterus. To our knowledge, this is the first time that an Ang antibody recognizes a band above 55 kD. This antibody (and the ang-1 antibody, GT28451) give a band at 75 kD which resembles the original size, because these are soluble highly glycosylated proteins, which should run higher that the calculated molecular weight of 55 kD.
|0.02M potassium phosphate with 0.15M NaCl and 0.1% sodium azide; pH 7.2|
|Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot|
|synthetic peptide amino acids 21-40 of Mouse Angiopoietin 2.|
|Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
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