Cytochrome C Mouse anti-Human, Invitrogen
Manufacturer: Invitrogen 459160
ApoTrack Cytochrome c WB Cocktail (SkuNo. 459160) provides a means to detect apoptosis while detecting release of cytochrome c, as well as a cytoplasmic and mitochondrial markers with the help of western blot. This cocktail provides a means to detect cytochrome c, as well as a cytoplasmic and mitochondrial markers Antibodies Cocktail Contains: Mouse anti Cyt. c monoclonal: Amount: 50 µg Working concentration: 1 µg/mL Mouse anti GAPDH monclonal: Amount: 5 µg Working concentration: 0.1 µg/mL Mouse anti PDH-E1-alpha monoclonal: Amount: 100 µg Working concentration: 2 µg/mL Mouse anti C-V-alpha monoclonal: Amount: 25 µg Working concentration: 0.5 µg/mLCytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.
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|HEPES buffered saline with 0.02% sodium azide|
|CYC, HCS, THC4, cytochrome c|
|4° C, do not freeze|
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