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AHR Rabbit anti-Mouse, Rat, Polyclonal, Invitrogen™

Rabbit Polyclonal Antibody

Manufacturer:  Invitrogen PA1515

Catalog No. PA1515


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Description

Description

PA1-515 detects Ah receptor nuclear translocator (ARNT) from mouse and rat cells. PA1-515 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~87 kDa protein representing ARNT from Hepa 1c1c7 cell lysate. PA1-515 reacts with one other band lower than ARNT whose identity has not been established. PA1-515 immunizing peptide corresponds to amino acid residues 771-789 from human ARNT. This sequence is completely conserved between human and mouse ARNT.

AHR (Ah Receptor) belongs to a family of proteins comprised of its dimerization partner ARNT (HIF-1 Beta) and the Drosophila proteins PER and SIM. AHR contains an N-terminal sequence of approximately 200 amino acids termed the PAS domain. AHR, found in a variety of tissues, binds to a specific DNA enhancer sequence and initiates transcription of the mRNA for the cytochrome P-450 (CYPIA1) gene. The gene for AHR encodes a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. AHR has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450, and its ligands included a variety of aromatic hydrocarbons. AHR is a ligand-activated helix/loop/helix transcription factor found in a variety of vertebrate species. The known ligands for AHR are foreign planar aromatic compounds, such as polycyclic aromatic compounds and halogenated aromatic compounds such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Unlike the steroid/thyroid hormone receptors, there is no known physiological ligand for AHR. Studies indicate that in non-ligand activated cells, AHR is found complexed with HSP90 predomitly in the cytoplasm. Upon binding to an agonist, the ligand-activated AhR is believed to transform to a nuclear, DNA binding form, and this transformation process appears to involve dissociation of HSP90 from AhR followed by formation of a heterodimer with AhR nuclear translocator protein (Arnt). Diseases associated with AHR include eosinophilic fasciitis and seborrheic dermatitis.
Specifications

Specifications

AHR
Polyclonal
Unconjugated
Ahr
Liquid
AHR
Synthetic Peptide: C N(771) S Y N N E E F P D L T M F P P F S E(789)
Antigen affinity chromatography
RUO
Antibody
Polyclonal
Mouse, Rat
Immunocytochemistry, Immunofluorescence, Western Blot
0.6 mg/mL
PBS with 1mg/mL BSA and 0.05% sodium azide
P30561, P41738
Ah Receptor Nuclear Translocator, Aryl hydrocarbon receptor nuclear translocator, Dioxin receptor, nuclear translocator, Hypoxia-inducible factor 1 be
Rabbit
IgG
100 μL
-20° C, Avoid Freeze/Thaw Cycles
Primary
11622, 25690
Documents
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