ATM Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Thermo Scientific PA133016

Catalog No. PIPA133016

Description

PA1-33016 detects ATM from human, non-human primate, mouse samples. PA1-33016 has been successfully used in immunohistochemistry (paraffin tissue), Western blot applications. The PA1-33016 immunogen is: Recombit human ATM fragment (amino acids 1980-2338). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.

Ataxia-telangiectasia Mutated (ATM) is a protein that belongs to the PI3/PI4 kinase family. Ataxia-telangiectasia is a rare autosomal recessive disorder characterized by progressive neurologic degeneration, immunologic deficiency, and an increased risk of lymphoid cancer. The ATM gene codes for a protein belonging to the phosphoinositide 3-kinase (PI3K) superfamily. ATM phosphorylates proteins instead of lipid and has many downstream targets that act as cell-cycle regulators including: P53, Mdm2, BRCA1, and SMC1. The ATM protein is responsible for repairing double-stranded DNA breaks that occur because of ionizing radiation and other mutagens. The ATM’s C-terminal region has extensive homology to the catalytic domains of phosphatidylinositol 3-kinases (PI3 kinases). Studies have shown that ATM becomes autophosphorylated and upregulated by exposure to ionizing radiation. AT cells are hypersensitive to ionizing radiation, impaired in mediating the inhibition of DNA synthesis and display delays in p53 induction. Further, DNA damage caused by ionizing irradiation activates ATM-kinase, leading to a cascade of kinase reactions that regulate cell cycle, apoptosis, and DNA damage repair. Studies have linked ATM to apoptosis along with Nbs1 and Chk2 in the E2F1 pathway.

Specifications

• Antigen: ATM
• Classification: Polyclonal
• Gene: ATM
• Gene Alias: AT1, ATA, ATC, ATD, ATDC, ATE, DKFZp781A0353, MGC74674
• Host Species: Rabbit
• Quantity: 50 μL
• Primary or Secondary: Primary
• Target Species: Hamster, Human, Mouse, Non-human Primate
• Product Type: Antibody
• Isotype: IgG

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Conjugate: Unconjugated
• Gene Accession No.: Q13315, Q62388
• Gene Symbols: ATM
• Immunogen: Recombit human ATM fragment (amino acids 1980-2338).
• Regulatory Status: RUO
• Gene ID (Entrez): 11920, 472
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid