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BrdU Mouse anti-Chemical, Clone: MoBU-1, Invitrogen™

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen MA119213

Catalog No. 01-675-977


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Description

Description

This antibody reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle, and it reacts also specifically with 5-bromouridine (BrU).

Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.
Specifications

Specifications

BrdU
Monoclonal
1 mg/mL
PBS with 15mM sodium azide; pH 7.4
5-Bromo-2-deoxyuridine; Bromodeoxyuridine
BrdU conjugated to hemocyanine.
Protein A
RUO
Antibody
Monoclonal
Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin)
MoBu-1
Unconjugated
Liquid
Mouse
IgG1
100 μg
4° C, do not freeze
Primary
Chemical
Documents
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