Bub1 Rabbit anti-Human, Mouse, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA520381
A suggested positive control is A-20 cell lysate. PA5-20381 can be used with blocking peptide PEP-0498.Bub1 was initially identified as the mammalian homolog to the yeast protein through a cDNA screen. Bub1 encodes a kinase involved in the formation of the spindle checkpoint, an important mechanism that ensures high fidelity mitotic chromosome segregation. It is thought to be required for assembly of a functional inner centromere, sister chromatid cohesion via targeting of the Shugoshim protein and metaphase congression. Bub1 functions by phosphorylating cdc20, a member of the mitotic checkpoint complex and activating the spindle checkpoint. A related protein kinase Bub3 interacts with Bub1 and targets it to kinetochores prior to chromosome alignment. Mutations in bub1 have been associated with aneuploidy and several forms of cancer.
|A 19 amino acid peptide from near the amino terminus of human Bub1.|
|Antigen affinity chromatography|
|Immunocytochemistry, Immunofluorescence, Western Blot|
|PBS with 0.02% sodium azide|
|BUB1A, BUB1L, hBUB1; BUB1 budding uninhibited by benzimidazoles 1 homolog; mitotic checkpoint serine/threonine-protein kinase BUB1; mitotic spindle checkpoint kinase; putative serine/threonine-protein kinase|
|Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
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