CA150 Mouse, Unlabeled, Clone: 5, BD
Mouse Monoclonal Antibody
Manufacturer: BD Biosciences 611004
Human immunodeficiency virus type 1 (HIV-1) promoter activation requires the binding of Tat (transactivator protein) and other coactivator proteins to viral RNA elements such as TAR (transactivation response element). Interactions between Tat and host factors may serve to regulate HIV-1 transcription. CA150 (Coactivator 150kDa), a nuclear protein that associates with human RNA polymerase II holoenzyme, is a transcriptional coactivator of Tat. CA150 contains a glutamine and alanine rich repeat (QA), a feature of transcriptional activators, and proline rich and WWP regions that function in protein-protein interactions. In addition, CA150 contains a putative leucine zipper and a nuclear localization sequence (NLS). In vitro depletion of CA150 abrogated Tat mediated transactivation, while over-expression of a CA150 mutant lowered Tat-mediated activation in vivo. In addition, both CA150 and Tat coimmunoprecipitate with RNA polymerase II holoenzyme. These results suggest that CA150 is a transcriptional coactivator that associates with RNA polymerase II holoenzyme and, in turn, is involved in Tat-mediated transcription of the HIV-1 promoter.
Immunofluorescence, Western Blotting
|Canine, Human, Murine, Rat|
|Immunofluorescence, Western Blot|
|Human CA150 aa. 568-678|
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