Calbindin D28K Rabbit anti-Human, Polyclonal, Invitrogen™
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA137206
Calbindin-D-28K (also termed vitamin D-dependent calcium-binding protein, or cholecalcin), is a highly conserved 28kDa calcium binding protein, with broad tissue distribution. It belongs, together with calmodulin, S-100, parvalbumin, trponin C and other proteins, to a family of low molecular weight calcium binding proteins (CaBPs). These CaBPs have homologous primary structures, which contain conserved polypeptide folds of the EF-hand type for Ca2+ binding. Calbindin-D-28K is found predominantly in subpopulations of central and peripheral nervous system neurons, and in certain epithelial nervous system neurons, and in certain epithelial cells involved in Ca2+ transport such as distal tubular cells and cortical collecting tubules of the kidney, and in enteric neuroendocrine cells.This antibody is predicted to react with bovine, chicken, mouse and rat based on sequence homology.Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is kidney or cerebellum tissue.
|Synthetic peptide derived from C-terminus of human calbindin D28K protein.|
|Antigen affinity chromatography|
|Immunohistochemistry (Paraffin), Western Blot|
|PBS with 1% BSA and <0.1% sodium azide; pH 7.6|
|CALB, D-28K, RTVL-H protein, calbindin, calbindin 1, 28kD, calbindin D28, vitamin D-dependent calcium-binding protein, avian-type|
|4° C, do not freeze|
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