Invitrogen™ Calcineurin B Monoclonal Antibody (212306)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA523933

Catalog No. PIMA523933

Description

This antibody detects endogenous human, mouse, and rat Calcineurin B. Reconstitute at 0.5 mg/mL in sterile PBS.

Calcineurin is a Ser/Thr, calcium and calmodulin-dependent protein phosphatase that plays an essential role in the T cell activation pathway. Calcineurin (also referred to as protein phosphatase 2B) is composed of two subunits; calcineurin A (CnA), a 60 kDa catalytic subunit which is highly homologous to protein phosphatases 1 and 2A; and a 19 kDa regulatory subunit, calcineurin B (CnB) which, like calmodulin, contains four EF-hand calcium binding sites. Calcineurin-mediated dephosphorylation of the nuclear factor of activated T-cells (NF-AT) is essential for NF-AT activation, nuclear translocation, and early gene expression in T-cells. The immunosuppressive drugs, cyclosporin A and FK506, when bound to the immunophilins, cyclophilin and FKBP respectively, inhibit dephosphorylation and activation of NF-AT by calcineurin. This inhibition is caused by the immunosuppressant/immunophilin complex simultaneously binding to both CnA and CnB which does not inhibit the phosphatase activity of CnA, but rather blocks access of the substrate to the catalytic domain of CnA.

Specifications

• Antigen: Calcineurin B
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 5% trehalose and No Preservative
• Gene Accession No.: P63098, P63100, Q63810
• Gene Symbols: PPP3R1
• Immunogen: E. coli-derived recombinant human Calcineurin B Met1-Val170.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG2a

• Applications: Western Blot
• Clone: 212306
• Conjugate: Unconjugated
• Gene: PPP3R1
• Gene Alias: ALNB1; calcineurin B, type I (19kDa); calcineurin subunit B type 1; CALNB1; CaNB1; Cna2; Cnb; Cnb1; MCIP1; PP2B beta 1; PPP3R1; protein phospatase 3, regulatory subunit B, alpha isoform (calcineurin B, type I); protein phospatase 3, regulatory subunit B, alpha isoform,type 1; protein phosphatase 2B regulatory subunit 1; protein phosphatase 2B regulatory subunit B alpha; protein phosphatase 3 (formerly 2B), regulatory subunit B (19kD), alpha isoform (calcineurin B, type I); protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform (calcineurin B, type I); protein phosphatase 3 (formerly 2B), regulatory subunit B, alpha isoform; protein phosphatase 3 regulatory subunit B alpha; Protein phosphatase 3 regulatory subunit B alpha isoform 1; protein phosphatase 3 regulatory subunit B, alpha; protein phosphatase 3, regulatory subunit B, alpha; protein phosphatase 3, regulatory subunit B, alpha isoform (calcineurin B, type I)
• Host Species: Mouse
• Purification Method: Protein A/G
• Regulatory Status: RUO
• Gene ID (Entrez): 19058, 29748, 5534
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Lyophilized