MilliporeSigma™ anti-Calreticulin Clone: FMC75,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ 20891250UG

Catalog No. 20-891-250UG

Description

Specifically detects Calreticulin Clone: FMC75 in Human, Monkey samples, and it is validated for Enzyme Immunoassay, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immuno Blotting

Calreticulin is a calcium binding protein found primarily in the ER and the sarcoplasmic reticulum, although it also has been detected in the nucleus and nuclear envelope. It has the conserved KDEL sequence at its C-terminus for retention in the ER. It contains both high and low affinity calcium binding sites, is involved in calcium storage and regulation. The calcium binding at the C-terminus of calreticulin has been postulated to be involved with regulating the interaction of KDEL proteins with the KDEL receptor. Calreticulin also acts as a molecular chaperone for protein folding, in concert with Grp78 and Grp94.

Specifications

• Antigen: Calreticulin
• Classification: Monoclonal
• Conjugate: Unconjugated
• Host Species: Mouse
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Human, Monkey
• Form: Purified

• Applications: ELISA, Flow Cytometry, Immunoblot, Immunoblot, Immunocytochemistry, Immunohistochemistry
• Clone: FMC75
• Formulation: In PBS, 0.1mM PMSF, 50% glycerol, pH 7.2.
• Immunogen: a recombinant protein consisting of human calreticulin fused to maltose binding protein
• Regulatory Status: RUO
• Test Specificity: Recognizes the ∽63kDa (apparent MW) calreticulin protein in Molt 4, MCF-7, HeLa, K562, A431, Jurkat, Hs67, Hs198, and vero cells.
• Content And Storage: −20°C
• Isotype: IgG1

For Research Use Only