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Calretinin Rabbit anti-Human, Mouse, Polyclonal, Invitrogen™

Rabbit Polyclonal Antibody

Manufacturer:  Invitrogen PA137223

Catalog No. 01-673-149


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Description

Description

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is mesothelioma.

Recognizes a protein of 31.5kDa, identified as Calretinin. Calretinin is an intracellular calcium-binding protein belonging to the troponin C superfamily characterized by a structural motif described as the EF-hand domain. The immunohistochemical detection of calretinin in developing cerebellum is restricted to the later stages indicated by weak staining from week 21 of gestation, in Purkinje and basket cells and in neurons of the dentate nucleus. The intensity of staining increases as the cerebellum matures. In tumors, calretinin has been detected in mesotheliomas and some pulmonary adenocarcinomas.
Specifications

Specifications

Calretinin
Polyclonal
PBS with 1% BSA and 0.1% sodium azide; pH 7.6
P22676, Q08331
CAB29, CAL2, CR, 29 kDa calbindin, calbindin 2, 29kD, calretinin, calbindin D29K, calretinin
Rabbit
IgG
1 mL
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Primary
12308, 794
Immunohistochemistry (Paraffin), Western Blot
Unconjugated
CALB2
Liquid
CALB2
Recombit full length mouse calretinin protein.
Antigen affinity chromatography
RUO
Antibody
Polyclonal
Human, Mouse
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