Calretinin Rabbit anti-Human, Mouse, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA137223
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is mesothelioma.Recognizes a protein of 31.5kDa, identified as Calretinin. Calretinin is an intracellular calcium-binding protein belonging to the troponin C superfamily characterized by a structural motif described as the EF-hand domain. The immunohistochemical detection of calretinin in developing cerebellum is restricted to the later stages indicated by weak staining from week 21 of gestation, in Purkinje and basket cells and in neurons of the dentate nucleus. The intensity of staining increases as the cerebellum matures. In tumors, calretinin has been detected in mesotheliomas and some pulmonary adenocarcinomas.
|PBS with 1% BSA and 0.1% sodium azide; pH 7.6|
|CAB29, CAL2, CR, 29 kDa calbindin, calbindin 2, 29kD, calretinin, calbindin D29K, calretinin|
|Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Immunohistochemistry (Paraffin), Western Blot|
|Recombit full length mouse calretinin protein.|
|Antigen affinity chromatography|
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