Calsequestrin Mouse, Unlabeled, Clone: 51, BD
Mouse Monoclonal Antibody
Manufacturer: BD Biosciences 611652
Calsequestrin, a Ca2+-binding protein, is located in the lumen of the sarcoplasmic reticulum (SR) in cardiac and skeletal muscle. It binds Ca2+; with high capacity (40-50 mol Ca2+;/mol of protein) and low affinity (KD = 1mM). In the SR, calsequestrin retains large amounts of Ca2+; at locations near the ryanodine receptor/Ca2+;-release channels, which may act as a Ca2+;+-storage depot for rapid release of Ca2+; through these channels. Electron microscopy shows that calsequestrin is connected to Ca2+;-release channels via a network of anchoring filaments. These anchoring filaments contain two membrane spanning proteins called triadin and junctin, which are found in the SR. Both triadin and junctin have mixed charged clusters (KEKE motifs) that facilitate binding to both calsequestrin and ryanodine receptors. In addition, interaction between triadin and calsequestrin is enhanced at low Ca2+; concentrations. Thus, the quaternary anchoring complex that includes calsequestrin, ryanodine receptor, junctin, and triadin facilitates flow of Ca2+; ions from calsequestrin to the ryanodine receptor and concentrates Ca2+; ions at the inner face of the junctional SR membrane.
Host Species: Mouse
Species Reactivity [for Features Main]: Rat
Immunogen: Mouse Calsequestrin aa. 282-389
Immunofluorescence, Western Blotting
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