Carbonic Anhydrase IX/CA9 Antibody - BSA Free, Novus Biologicals™

Rabbit Polyclonal Antibody

Catalog Number	Mfr. No.	Quantity
NB100417SS	Novus Biologicals NB100417SS	0.025 mL
NB100417	Novus Biologicals NB100417	0.1 mL

Description

Carbonic Anhydrase IX/CA9 Polyclonal specifically detects Carbonic Anhydrase IX/CA9 in Human, Mouse, Rat, Plant samples. It is validated for Western Blot, Simple Western, Chromatin Immunoprecipitation, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunoblotting, Gel Super Shift Assays, Proximity Ligation Assay, Microarray, Dual RNAscope ISH-IHC.

Specifications

• Antigen: Carbonic Anhydrase IX/CA9
• Dilution: Western Blot 1 - 3 ug/ml, Simple Western 1:50, Flow Cytometry 1:1000, ELISA reported in scientific literature (PMID 19963243), Immunohistochemistry 1:200 - 1:500, Immunocytochemistry/Immunofluorescence 2 - 5 ug/ml, Immunoprecipitation 1:10 - 1:500. Use reported in multiple pieces of scientific literature, Immunohistochemistry-Paraffin 1:200 - 1:500, Immunohistochemistry-Frozen 1:200 - 1:500, Immunoblotting, Gel Super Shift Assays, Proximity Ligation Assay, Microarray reported in scientific literature (PMID 31955345), Chromatin Immunoprecipitation (ChIP) 1:10-1:500, Dual RNAscope ISH-IHC 1:1000
• Classification: Polyclonal
• Host Species: Rabbit
• Research Discipline: Cancer, Cellular Markers, HIF Target Genes, Hypoxia, Lipid and Metabolism, Signal Transduction
• Formulation: PBS with 0.02% Sodium Azide
• Gene Alias: CA-IX, CAIXcarbonic anhydrase 9, Carbonate dehydratase IX, carbonic anhydrase IXpMW1, carbonic dehydratase, EC 4.2.1.1, G250, Membrane antigen MN, MNRCC-associated antigen G250, P54/58N, RCC-associated protein G250, Renal cell carcinoma-associated antigen G250
• Gene Symbols: CA9
• Isotype: IgG
• Purification Method: Affinity Purified
• Test Specificity: Human, dog, rat and mouse (PMID 22842475).

• Concentration: 1.0 mg/mL
• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunoblot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), In Situ Hybridization (ISH), In situ PLA
• Conjugate: Unconjugated
• Regulatory Status: RUO
• Target Species: Human, Mouse, Rat, Canine, Plant
• Gene Accession No.: Q16790
• Gene ID (Entrez): 768
• Immunogen: A synthetic peptide made to a C-terminal sequence of human Carbonic Anhydrase IX (within residues 400-459) [UniProt Q16790]
• Primary or Secondary: Primary
• Content And Storage: Store at -20C. Avoid freeze-thaw cycles.
• Molecular Weight of Antigen: 55 kDa

For Research Use Only