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Caspase 1 Rabbit anti-Human, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA137232
This antibody is predicted to react with bovine, canine, mouse, porcine and rat based on sequence homology. Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is placenta tissue.Caspase-1/ICE (Interleukin-1Beta Converting Enzyme) is expressed as a proenzyme of 45kDa in many tissues. Upon stimulus, it gets activated by proteolytic cleavage. Active caspase-1 is a tetramer of two subunits p10 and p20 in 2; 2 proportion. Caspase-1 (cystein aspartic-specific protease 1) cleaves its substrates after an aspartate residue. It cleaves Interleukin Beta into its active form which further exerts its proinflammatory effects. Overexpression of caspase-1 can induce apoptosis in fibroblast, which can be inhibited by overexpression of Crm A, a protein from pox virus.
|PBS with 1% BSA and 0.1% sodium azide; pH 7.4|
|ICE, IL1BC, P45, CASP1 nirs variant 1, IL-1 beta-converting enzyme, IL1B-convertase, caspase 1, apoptosis-related cysteine peptidase interleukin 1, beta, convertase, caspase-1, interleukin 1, beta, convertase, interleukin 1-B converting enzyme|
|-20° C, Avoid Freeze/Thaw Cycles|
|Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot|
|Synthetic peptide derived from internal region of human caspase 1 protein|
|Antigen affinity chromatography|
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