beta Catenin Rabbit anti-Chicken, Human, Mouse, Rat, Xenopus laevis, Polyclonal, Invitrogen™
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA137255
This antibody is predicted to react with bovine, amphibian, mouse and rat based on sequence homology. Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is breast carcinoma.Beta-catenin, an adherens junction (AJ) protein, was originally identified as a component of cell-cell adhesion structures. AJs are necessary for the creation and maintece of epithelial cell layers by regulating cell growth and adhesion between cells. Beta-catenin interacts with the cytoplasmic domain of E-cadherin and links E-cadherin to alpha-catenin, which in turn mediates anchorage of the E-cadherin complex to the cortical actin cytoskeleton. Studies show that Beta-catenin also binds to another cytoskeletal complex containing the adenomatous polyposis coli protein and microtubules, and interacts with several signaling pathways that include tyrosine kinases, phosphatases and Wnt/Wingless. The interplay between beta-catenin, cytoskeletal complexes and signaling pathways may regulate morphogenesis. Beta-catenin is expressed in several hair follicle cell types, basal and peripheral matrix cells, and cells of the outer and inner root sheats. A pathological role of beta-catenin has been identified in pilomatrixoma (PTR), medulloblastoma (MDB), colorectal cancer (CRC), ovarian cancer, and tumor development. In the nucleus, beta-catenin serves to co activate a family of Lef/Tcf transcription factors that stimulate transcription of target genes including those encoding cyclin D and c-myc that promote cell proliferation. The influence on cell proliferation is the molecular basis for the role of beta-catenin in tumorgenesis, specifically, solid tumors of the breast, colon, liver, lung, gastric, prostate, and skin.
|PBS with 1% BSA and 0.1% sodium azide; pH 7.6|
|Q02248, P26233, P35222, Q9WU82|
|Cadherin associated protein beta, Catenin beta 1, CATNB, CTNNB|
|Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|84353, 1499, 399274, 395964, 12387|
|Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot|
|Synthetic peptide from the C-terminus of the catenin-beta protein|
|Antigen affinity chromatography|
|Chicken, Human, Mouse, Rat, Xenopus laevis|
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