CD138 Rat anti-Mouse, BUV737, Clone: 281-2, BD
Rat Monoclonal Antibody
Manufacturer: BD Biosciences 564430
The 281-2 monoclonal antibody specifically binds to the core protein of CD138 (Syndecan-1), a cell-surface, integral membrane heparan sulfate- and chondroitin sulfate-containing proteoglycan that binds to interstitial extracellular matrix molecules. Syndecan-1 is predominantly expressed on epithelial cells, where its expression correlates with normal epithelial organization. It is also expressed on B lymphocytes at specific stages during their differentiation: precursor B cells in the bone marrow and antibody-secreting cells, including plasma cells, but not mature peripheral B cells. It is thus implicated in mediating B cell-matrix interactions. CD138 expression is also regulated during embryonic development, and the molecule shows a tissue- specific structural polymorphism resulting from different post-translational modifications. The 281-2 antibody may be used to detect the differently glycosylated forms, because it reacts with the core protein. Furthermore, the mAb detects the Syndecan-1 ectodomain which is cleaved from cell surfaces by a metalloproteinase.
The antibody was conjugated to BD Horizon BUV737 which is part of the BD Horizon Brilliant Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 737-nm. BD Horizon Brilliant BUV737 can be excited by the ultraviolet laser (355 nm) and detected with a 740/35 filter. Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into channels detecting Alexa Fluor 700-like dyes (eg, 712/20-nm filter).
|Brilliant Ultraviolet 737|
|Aqueous buffered solution containing ≤0.09% sodium azide|
|SYND1; Syndecan-1; syn-1; Sdc1; Sstn; synstatin|
|NAMRU mouse mammary gland epithelial cell line NMuMG|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
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