CD140b (pY1021) Mouse anti-Human, PE, Clone: J105-412, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 558417

Catalog No. BDB558417

Description

Platelet-derived growth factor (PDGF) is a potent mitogen for cells of mesenchymal origin and exerts its effects by binding to the PDGF receptor (PDGFR), a transmembrane protein tyrosine kinase. PDGFR is composed of PDGFRα (CD140a) and/or PDGFRβ (CD140b) polypeptides. Both PDGF and PDGFR consist of subunits that form homo- or heterodimers with varying specificities: PDGF-AA binds only to αα PDGFR, PDGF-AB binds to both αα and αβ PDGFR, and PDGF-BB binds to all three PDGFRs . Ligand binding induces dimerization and activation of the receptor. Upon activation, CD140b is phosphorylated at multiple tyrosine sites and, in turn, an intracellular phosphorylation cascade is initiated. PDGFR localizes primarily to membrane invagin ations termed caveolae, compartments that are enriched in several of its downstream effectors, including phosphatidylinositol 3'-kinase, Src, and phospholipase C-γ(PLC-γ).

The J105-412 monoclonal antibody recognizes the phosphorylated tyrosine 1021 (pY1021 ) in the C-terminal noncatalytic region of CD140b, which interacts primarily with PLC-γ. The orthologous phosphorylation site in mouse PDGFRβ is Y1020.

Specifications

• Antigen: CD140b (pY1021)
• Classification: Monoclonal
• Conjugate: PE
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Immunogen: Phosphorylated Human PDGFRβ Peptide
• Quantity: 50 Tests
• Research Discipline: Cell Biology
• Target Species: Human
• Isotype: IgG1 κ

• Applications: Flow Cytometry
• Clone: J105-412
• Description: PDGF Receptor β
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.