The W6D3 monoclonal antibody specifically binds to 3-fucosyl-N-acetyllactosamine (3-FAL), a 220 kDa carbohydrate structure, also called X-hapten. 3-FAL is expressed on >95% of granulocytes, including neutrophils and eosinophils, and on monocytes to a varying degree, but not on lymphocytes or basophils. CD15 plays a role in mediating phagocytosis, bactericidal activity and chemotaxis. Most CD15 antibodies are IgM isotype; clone W6D3 is a mouse IgG1 isotype. In comparison studies with clone HI98, a known CD15 antibody, clone W6D3 shows brighter fluorescence staining and its binding can be blocked by clone HI98.
This antibody is conjugated to BD Horizon BV605 which is part of the BD Horizon Brilliant Violet family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.
|Aqueous buffered solution containing BSA and ≤0.09% sodium azide.|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
|Brilliant Violet 605|
For Research Use Only.
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