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CD15 Mouse anti-Human, Brilliant Violet 650, Clone: HI98 (also known as HIM1), BD

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 564232

Catalog No. BDB564232

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The HI98 monoclonal antibody specifically reacts with 3-fucosyl-N-acetyllactosamine (3-FAL), a 220 kDa carbohydrate structure, also called X-hapten, SSEA-1, CD15 or Lewis X. This structure is found on a variety of cell surface glycolipids and glycoproteins. 3-FAL is expressed on >95% of granulocytes, including neutrophils and eosinophils, and to a varying degree on monocytes, but not on lymphocytes or basophils. CD15 plays a role in mediating phagocytosis, bactericidal activity and chemotaxis. This antibody is also suitable for staining formalin-fixed, paraffin-embedded tissue sections without pretreatment. Since the Abs are recognizing a carbohydrate epitope (3-fucosyl-N-acetyllactosamine) they also should work across species and not only for human.

The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.



HI98 (also known as HIM1)
Lewis X; Le-X; X-Hapten; SSEA-1; 3-FAL
Affinity Purified
Human Mononuclear Cells
100 Tests
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Flow Cytometry
Brilliant Violet 650
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
IgM, κ
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