CD172a/b Mouse anti-Human, Brilliant Violet 421, Clone: SE5A5, BD
Mouse Monoclonal Antibody
Manufacturer: BD Biosciences 565036
The SE5A5 monoclonal antibody specifically binds to a common epitope on CD172a/ SIRPα (90 kDa) and CD172b/ SIRPβ 1(50 kDa) . These transmembrane glycoproteins are members of the Signal Regulatory Protein (SIRP) family that, in turn, belongs to the Immunoglobulin superfamily. The SIRP family is comprised of two subgroups, SIRPα and SIRPβ that are distinguished by the presence (α) or absence (β) of a cytoplasmic domain containing two immunoreceptor tyrosine-based inhibition motifs (ITIM). CD172a/ SIRPα is expressed on CD34+ stem/progenitor cells, cardiomyocytes, monocytes, macrophages, granulocytes, dendritic cells, and in the central nervous system. It binds to CD47 and is implicated in mediating inhibitory signals via the ITIM/SHP-2 association. CD172b/ SIRPβ to cellular activation signaling . CD172b is expressed on myeloid cells, including peripheral blood monocytes and granulocytes. It is not expressed on CD34+ cells. CD172a and CD172b have complementary role s in signal regulation and may work together in tuning certain cellular responses to stimuli.
The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.
|SIRP alpha/beta1; SIRPα/SIRPβ1; Signal Regulatory Protein α/β1|
|Human SIRP alpha extracellular domain Recombinant Protein|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
|Brilliant Violet 421|
|Aqueous buffered solution containing BSA and ≤0.09% sodium azide|
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