CD22 Rabbit anti-Human, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA527928
PA5-27928 targets CD22 in WB applications and shows reactivity with Human samples. The PA5-27928 immunogen is recombit fragment corresponding to a region within amino acids 193 and 710 of Human CD22. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.CD22 (BL-CAM) is a type 1 integral membrane glycoprotein with molecular weight of 130 to 140 kDa. CD22 is expressed in both the cytoplasm and cell membrane of B-lymphocytes. CD22 antigen appears early in B-cell lymphocyte differentiation at approximately the same stage as the CD19 antigen. Unlike other B-cell markers, CD22 membrane expression is limited to the late differentiation stages comprised between mature B cells (CD22+) and plasma cells (CD22-), and may thus prove useful in phenotyping mature leukemias. CD22 is also strongly expressed in hairy cell leukemia. CD22 preferentially binds to alpha2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B cell antigen receptor signaling. CD22 plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. CD22 is also strongly expressed in hairy cell leukemia. CD22 is also positive in diffuse large B-cell lymphoma and nodular lymphocyte predomice Hodgkin’s lymphoma, but negative in classical Hodgkin’s lymphoma.
|Recombit fragment corresponding to a region within amino acids 193 and 710 of Human CD22|
|Antigen affinity chromatography|
|0.1M tris glycine with 10% glycerol and 0.01% thimerosal; pH 7|
|FLJ22814, MGC130020, SIGLEC-2, SIGLEC2, B-cell receptor CD22, B-lymphocyte cell adhesion molecule, BL-CAM, CD22 antigen, T-cell surface antigen Leu-14, sialic acid binding Ig-like lectin 2, sialic acid-binding Ig-like lectin 2|
|Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
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