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CD3 Mouse anti-Human, BB515, Clone: HIT3a, BD

Mouse Monoclonal Antibody

Specifications

Antigen CD3
Isotype IgG2a, κ
Formulation Aqueous buffered solution containing ≤0.09% sodium azide
Conjugate BB515
Primary or Secondary Primary
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Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
BDB565101
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BD Biosciences
565101
25 Tests
Each for $66.50
N/A
BDB565100
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BD Biosciences
565100
100 Tests
Each for $138.40
N/A
Description

Description

The HIT3a monoclonal antibody specifically binds to the human CD3 ε-chain, a 20 kDa subunit of the CD3/T cell antigen receptor complex found on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes. Studies from the HLDA Workshop show that this antibody can be mitogenic for T lymphocytes. The CD3 complex plays a role in signal transduction during antigen recognition by the T cell receptor. HIT3a antibody does not stain intracellular CD3 unlike the other CD3-specific clone, UCHT1 (Cat. No. 555330/550368).

The antibody was conjugated to BD Horizon BB515 which was developed exclusively by BD Biosciences. With an excitation max of 490nm and an emission max of 515nm, BD Horizon BB515 can be excited by the 488nm laser and detected in a standard FITC set (eg, 530/30-nm filter). This dye provides a much brighter alternative to FITC with less spillover into the PE detector.

Specifications

Specifications

CD3
Aqueous buffered solution containing ≤0.09% sodium azide
Primary
Mouse
Affinity purified
Monoclonal
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
IgG2a, κ
BB515
Flow Cytometry
RUO
HIT3a
CD3E; T3E; TCRE; cd 3; cd-3; cd3; CD3-epsilon; 916
SDS
Product Certifications

For Research Use Only.

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