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CD3 Mouse anti-Human, Brilliant Violet 711, Clone: UCHT1, BD
Mouse Monoclonal Antibody
Supplier: BD Biosciences 563725
Description
The UCHT1 monoclonal antibody specifically binds to the human CD3 ε -chain, a 20-kDa subunit of the CD3/T cell antigen receptor complex. CD3 ε is expressed on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes. Studies from the HLDA Workshop show that this antibody is mitogenic for CD3 ε -positive cells when used in conjunction with costimulatory agents such as pokeweed mitogen or anti-CD28 antibody. CD3 plays a central role in signal transduction during antigen recognition. The UCHT1 antibody stains both surface and intracellular CD3 ε unlike the other CD3 clone, HIT3a, that stains only extracellular CD3 ε .
The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm. BD Horizon™ BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy™5.5 detectors. However, the spillover can be corrected through compensation as with any other dye combination.
Specifications
CD3 | |
Monoclonal | |
Brilliant Violet 711 | |
Aqueous buffered solution containing BSA and ≤0.09% sodium azide. | |
Human infant thymocytes and peripheral blood lymphocytes from a Sezary Syndrome donor | |
100 Tests | |
Primary | |
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
Flow Cytometry | |
UCHL1 | |
CD3e; CD3E; T3E; TCRE; T-cell surface antigen T3/Leu-4 epsilon | |
Mouse | |
Affinity Purified | |
RUO | |
Human | |
IgG1 κ |
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For Research Use Only.