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CD49e Rat anti-Mouse, PE-CF594, Clone: 5H10-27 (MFR5), BD

Rat Monoclonal Antibody

Manufacturer:  BD Biosciences 564313

Catalog No. BDB564313


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Description

Description

The 5H10-27 (MFR5) monoclonal antibody specifically binds to the α5 chain of the integrin α5β1 fibronectin receptor (CD49e/CD29, VLA-5) on thymocytes, activated T lymphocytes, precursor and activated B cells, mast cells, platelets, epithelial and endothelial cells, fibroblasts and a variety of mouse cell lines. Soluble 5H10-27 (MFR5) antibody has been reported to inhibit VLA-5-mediated functions in vitro. In addition, immobilized 5H10-27 (MFR5) antibody reportedly costimulates the proliferative response of CD8+ T cells to plate-bound anti-CD3e antibody.

This antibody is conjugated to BD Horizon PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to PE-Texas Red™. PE-CF594 excites and emits at similar wavelengths to PE-Texas Red™ yet exhibits improved brightness and spectral characteristics. Due to PE having maximal absorption peaks at 496nm and 564nm, PE-CF594 can be excited by the blue (488-nm), green (532-nm) and yellow-green (561-nm) lasers and can be detected with the same filter set as PE-Texas Red™ (eg 610/20-nm filter).

Specifications

Specifications

CD49e
5H10-27 (MFR5)
PE-CF594
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Rat
IgG2a, κ
RUO
Primary
Murine
Flow Cytometry
0.2mg/mL
Itga5; ITA5; Integrin alpha-5; Fibronectin receptor alpha; Fnra; VLA5 alpha
Affinity Purified
Mouse Mast Cell Line MC/9
100μg
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Monoclonal
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