MilliporeSigma™ anti-CD8a APC, Clone: 53-6.7,

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
MABF1535MI	MilliporeSigma™ MABF1535	25 μg
MABF1536MI	MilliporeSigma™ MABF1536	100 μg

Description

Specifically detects CD8a Clone: 53-6.7 in Mouse samples, and it is validated for Flow Cytometry

The 53-6.7 antibody reacts with the 32-34kDa alpha subunit of mouse CD8, known as CD8a or CD8 alpha. CD8a can form a homodimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor in antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck, facilitating intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by virus, bacteria or parasites.The 53-6.7 antibody is widely used as a phenotypic marker for mouse CD8a expression on cytotoxic T cells, thymocytes, as well as on certain cell types that do not also express the TCR, including some NK cells and lymphoid dendritic cells.

Specifications

• Antigen: CD8a
• Applications: Flow Cytometry
• Conjugate: APC
• Host Species: Rat
• Research Discipline: Inflammation, Immunology
• Formulation: Purified Rat monoclonal containing 10mM NaH2PO4, 150mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2.
• Gene Symbols: CD8a
• Isotype: IgG2a κ
• Purification Method: Purified by size-exclusion chromatography

• Clone: 53-6.7
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Mouse
• Gene Accession No.: P01731
• Immunogen: Proprietary immunogen
• Primary or Secondary: Primary
• Content And Storage: 2°C to 8°C undiluted for one year from date of shipment protected from light; Do not freeze

For Research Use Only