p14ARF Rabbit anti-Human, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA146021
By Western blot, PA1-46021 is specific for the ~16 kDa CDKN2A protein (CDKN2A tends to run slightly higher than the theoretical MW of 14 kDa). Additional non-specific bands may be seen at ~25 and 47 kDa. Suggested positive control: BT549 cells or HeLa whole cell extract, antigen standard for CDKN2A (transient overexpression lysate).The INK4a-ARF locus is comprised of two tumor suppressors, p16INK4a and p14ARF. These two proteins are encoded through differential splicing of alternative first exons. The p16INK4a (exon 1 alpha) protein inhibits the cyclin D-dependent kinases (CDK) that control the phosphorylation of the Rb protein and cell proliferation. The p14ARF gene product complexes with the MDM2 protein within the nucleus, thus modulating the activity of the p53 protein. P14ARF is a potent tumor suppressor in the presence of wild-type p53, while mutant p53 substantially reduces growth inhibition by p14ARF.
|A synthetic peptide made to a region within residues 100-173 of the human CDKN2A protein.|
|Antigen affinity chromatography|
|tris glycine with 0.15M NaCl and 0.05% sodium azide|
|ARF , CDK4 inhibitor p16INK4 , CDK4I , CDKN2 , CDKN2A , Cell cycle negative regu|
|-20°C or -80°C if preferred|
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