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STUB1 Rabbit anti-Human, Mouse, Polyclonal, Invitrogen™

Rabbit Polyclonal Antibody

Manufacturer:  Invitrogen PA1015

Catalog No. PA1015


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Description

Description

PA1-015 detects human and mouse carboxyl terminus of hsc70-interacting protein (CHIP). PA1-015 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~35 kDa protein representing CHIP from COS-1 cells overexpressing the human gene and a non-specific band at ~85 kDa. PA1-015 was used successfully in the western blot analysis of CHIP using human MCF7 cell lysates. The PA1-015 immunogen is a synthetic peptide corresponding to residues V(218) D E K R K K R D I P D Y L C(232) of human CHIP. This peptide (Cat. No. PEP-179) is available for use in neutralization and control experiments.

A recently identified protein, termed carboxyl terminus of hsc70-interacting protein (CHIP), has been shown to interact both with the constitutive form of hsc70 and the stress inducible form, hsp70. This novel 35 kDa cytoplasmic protein has been shown to be highly expressed in striated muscle in vivo. Additional studies have shown that this protein is expressed over a broad range of cultured tissues. Through immunoprecipitation experiments, CHIP has been shown to directly bind to the carboxyl terminus of hsc70 and hsp70 where it decreases ATPase activity and reduces overall chaperone efficiency.   CHIP has also been identified as an important protein in the ubiquitin-proteasome system. CHIP contains a U-box domain and acts as an E3 ubiquitin-ligase in conjunction with hsc70 and hsp90.

Abbreviations: AP=Affinity Purification, ChIP=Chromatin IP, CM=Confocal Microscopy, DB=Dot Blot, EIA=ELISA, EM=Electron Microscopy, FC=Flow Cytometry, GS=Gel Supershift, IC=Immunocytochemistry, IF=Immunofluorescence, IH=Immunohistology/Immunohistochemistry, IP=Immunoprecipitation, IPK=IP Kinase Assay, KA=Kinase Assay, NEUT=Neutralization, PIA=Peptide Inhibition Assay, RIA=Radioimmunossay, TCA=T Cell Activation, WB=Western Blotting, Species: Am=Amphibian, Av=Avian, B=Bovine, Ca=Cat ,Ck=Chicken, D=Dog, Dr=Deer, Ds=Drosophila, El=Elk, Fg=Frog, Fs=Fish, Ft=Ferret, G=Goat, Gp=Guinea Pig, H=Horse, Hm=Hamster, Hu=Human, K=Kangaroo, Ms=Mouse, Nhp=Non-Human Primate, O=Ovine, P=Porcine, Rb=Rabbit, Rp=Reptile, Rt=Rat, Ys=Yeast, Xn=Xenopus, (wk)=cross reacts weakly

Specifications

Specifications

STUB1
Polyclonal
Unconjugated
STUB1
Liquid
STUB1
Synthetic peptide corresponding to residues V(218) D E K R K K R D I P D Y L C(232) of human CHIP.
Antigen affinity chromatography
RUO
Antibody
Polyclonal
Human, Mouse
Western Blot
1 mg/mL
PBS with 1mg/mL BSA and 0.05% sodium azide
Q9UNE7, Q9WUD1
Carboxyl Terminus of Hsc70-Interacting Protein
Rabbit
IgG
100 μg
-20° C, Avoid Freeze/Thaw Cycles
Primary
10273, 56424
Documents
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