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anti-Chk1 Ab-1, Clone: DCS-300, Thermo Scientific Lab Vision

Mouse Monoclonal Antibody

Specifications

Antigen Chk1 Ab-1
Format Purified
Host Species Murine
Immunogen Recombinant human Chk1 protein.
Isotype IgG2b κ
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Products 6
Catalog Number Mfr. No. Concentration Conjugate Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Concentration Conjugate Quantity Price Quantity & Availability  
MS1513P1ABX
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Epredia
MS1513P1ABX
1mg/mL Unconjugated 100μL This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.
N/A
MS1513P0
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Epredia
MS1513P0
200μg/mL Unconjugated 100μL This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.
N/A
MS1513P
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Epredia
MS1513P
200μg/mL Unconjugated 1mL This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.
N/A
MS1513P1
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Epredia
MS1513P1
200μg/mL Unconjugated 500μL This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.
N/A
MS1513PABX
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Epredia
MS1513PABX
1mg/mL Unconjugated 200μL This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.
N/A
MS1513PCL
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Epredia
MS1513PCL
250μg/100μL Unconjugated 100μL This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.
N/A
Description

Description

Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both function as essential components in the G2-DNA damage checkpoint by phosphorylating CDC25C in response to DNA damage. CDC25A, CDC25B and CDC25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating CDC2 p34 on regulatory tyrosine residues. Phosphorylation of CDC25C by Chk1 and 2 inhibits its phosphatase activity, thereby blocking mitosis. It has also been shown that Chk1 can phosphorylate Wee1 in vitro, providing evidence that the hyperphosphorylated form of Wee1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1.

Host Species: Mouse
Clone: DCS-300
Isotype: IgG2b/κ
Cross Reactivity: Human. Others not known.
Immunogen: Recombinant human Chk1 protein.
Molecular Weight: ~56kDa
Positive Control: HeLa cells.
Cellular Localization: Nuclear

Western Blotting

Abbreviations: AP=Affinity Purification, ChIP=Chromatin IP, CM=Confocal Microscopy, DB=Dot Blot, EIA=ELISA, EM=Electron Microscopy, FC=Flow Cytometry, GS=Gel Supershift, IC=Immunocytochemistry, IF=Immunofluorescence, IH=Immunohistology/Immunohistochemistry, IP=Immunoprecipitation, IPK=IP Kinase Assay, KA=Kinase Assay, NEUT=Neutralization, PIA=Peptide Inhibition Assay, RIA=Radioimmunossay, TCA=T Cell Activation, WB=Western Blotting, Species: Am=Amphibian, Av=Avian, B=Bovine, Ca=Cat ,Ck=Chicken, D=Dog, Dr=Deer, Ds=Drosophila, El=Elk, Fg=Frog, Fs=Fish, Ft=Ferret, G=Goat, Gp=Guinea Pig, H=Horse, Hm=Hamster, Hu=Human, K=Kangaroo, Ms=Mouse, Nhp=Non-Human Primate, O=Ovine, P=Porcine, Rb=Rabbit, Rp=Reptile, Rt=Rat, Ys=Yeast, Xn=Xenopus, (wk)=cross reacts weakly

Specifications

Specifications

Chk1 Ab-1
Murine
IgG2b κ
Primary
Purified
Recombinant human Chk1 protein.
Monoclonal
SDS
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