anti-Chk1 Ab-1, Clone: DCS-300, Thermo Scientific Lab Vision

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Concentration	Conjugate	Quantity
MS1513P1ABX	Epredia MS1513P1ABX	1mg/mL	Unconjugated	100μL
MS1513P0	Epredia MS1513P0	200μg/mL	Unconjugated	100μL
MS1513P	Epredia MS1513P	200μg/mL	Unconjugated	1mL
MS1513P1	Epredia MS1513P1	200μg/mL	Unconjugated	500μL
MS1513PABX	Epredia MS1513PABX	1mg/mL	Unconjugated	200μL
MS1513PCL	Epredia MS1513PCL	250μg/100μL	Unconjugated	100μL

Description

Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both function as essential components in the G2-DNA damage checkpoint by phosphorylating CDC25C in response to DNA damage. CDC25A, CDC25B and CDC25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating CDC2 p34 on regulatory tyrosine residues. Phosphorylation of CDC25C by Chk1 and 2 inhibits its phosphatase activity, thereby blocking mitosis. It has also been shown that Chk1 can phosphorylate Wee1 in vitro, providing evidence that the hyperphosphorylated form of Wee1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1.

Host Species: Mouse
Clone: DCS-300
Isotype: IgG2b/κ
Cross Reactivity: Human. Others not known.
Immunogen: Recombinant human Chk1 protein.
Molecular Weight: ~56kDa
Positive Control: HeLa cells.
Cellular Localization: Nuclear

Western Blotting

Abbreviations: AP=Affinity Purification, ChIP=Chromatin IP, CM=Confocal Microscopy, DB=Dot Blot, EIA=ELISA, EM=Electron Microscopy, FC=Flow Cytometry, GS=Gel Supershift, IC=Immunocytochemistry, IF=Immunofluorescence, IH=Immunohistology/Immunohistochemistry, IP=Immunoprecipitation, IPK=IP Kinase Assay, KA=Kinase Assay, NEUT=Neutralization, PIA=Peptide Inhibition Assay, RIA=Radioimmunossay, TCA=T Cell Activation, WB=Western Blotting, Species: Am=Amphibian, Av=Avian, B=Bovine, Ca=Cat ,Ck=Chicken, D=Dog, Dr=Deer, Ds=Drosophila, El=Elk, Fg=Frog, Fs=Fish, Ft=Ferret, G=Goat, Gp=Guinea Pig, H=Horse, Hm=Hamster, Hu=Human, K=Kangaroo, Ms=Mouse, Nhp=Non-Human Primate, O=Ovine, P=Porcine, Rb=Rabbit, Rp=Reptile, Rt=Rat, Ys=Yeast, Xn=Xenopus, (wk)=cross reacts weakly

Specifications

• Antigen: Chk1 Ab-1
• Host Species: Murine
• Isotype: IgG2b κ
• Primary or Secondary: Primary

• Format: Purified
• Immunogen: Recombinant human Chk1 protein.
• Monoclonal or Polyclonal: Monoclonal