Claudin 4 Rabbit anti-Human, Mouse, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA137471
This antibody is predicted to react with bovine, canine, amphibian, mouse, porcine and rat based on sequence homology. Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is tonsil or ovarian carcinoma.Claudin-4 belongs to the 20-member family of claudins, transmembrane cellular adhesion proteins localized at epithelial and endothelial tight junctions. Claudins demonstrate distinct, tissue-specific expression patterns, and are essential components of tight junction charge-specific channels that regulate paracellular ion flux. Claudin-4 is a component of tight junction strands and may play a role in internal organ development and function during pre- and postnatal life. The overexpression of Claudin-4 has been shown to decrease paracellular electrical conductance due to a selective decrease in Na+ permeability, with no significant decrease in Cl- permeability. In ovarian carcinoma cells, Claudin-3 and Claudin-4 exhibit upregulation at both mRNA and protein levels. Claudin-4 is also overexpressed in pancreatic cancer tissues and cell lines, and in other epithelial cancers. The Claudin-4 gene is deleted in Williams-Beuren syndrome, a neurodevelopmental disorder affecting multiple systems.
|PBS with 1% BSA and 0.1% sodium azide|
|CPE-R, CPER, CPETR, CPETR1, WBSCR8, hCPE-R, CPE-receptor, Clostridium perfringens enterotoxin receptor 1, Williams-Beuren syndrome chromosomal region 8 protein, claudin-4|
|-20°C or -80°C if preferred|
|Immunohistochemistry (Paraffin), Western Blot|
|Synthetic peptide from the C-terminus of human claudin-4|
|Antigen affinity chromatography|
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