MilliporeSigma anti-Cleaved Bid (Ab-1), Polyclonal
Rabbit Polyclonal Antibody
Manufacturer: MilliporeSigma PC64510T
Specifically detects Cleaved Bid Clone: in Mouse samples, and it is validated for Immuno BlottingBid (~22kDa) is a BH3 domain containing pro-apoptotic cytosolic protein that maps to human chromosome 22q11.2. Initially localized to the cytoplasm, the Bid protein is cleaved by the action of caspase 8 into fragments of two different sizes, p15 and p13 and becomes inserted into the mitochondrial membrane. Of the two fragments the p15 BH3 containing fragment is the more potent inducer of apoptosis and in fact is a stronger inducer than the full-length Bid protein. Following cleavage by caspase 8, Bid will bind with Bax localizing it to the mitochondrial membrane and leading to the release of cyto-chrome c. This interaction is blocked by Bcl-x, which directly binds to Bax, competing for the binding of Bax to the membrane-localized Bid.
|a synthetic peptide corresponding to amino acids at the N-terminus of the 59/60 cleavage site of mouse Bid (BH3 interacting domain death agonist)|
|Immuno Blotting, Immunoblot|
|In Dulbecco's PBS (without Mg+2 and Ca+2), 1mg/mL BSA, 50% glycerol, pH 7.3.|
|Recognizes the ∽15kDa cleaved Bid protein. Does not recognize uncleaved, full-length Bid.|
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