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ROCK1 (Cleaved) Mouse anti-Canine, Human, Mouse, Non-human primate, Rabbit, Rat, Clone: 154C1465, Invitrogen™

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen MA141031

Catalog No. 01-676-444


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Description

Description

Suggested positive control: Jurkat cells treated with camptothecin or anti-Fas antibody to induce apoptosis..

The Rho target protein, Rho-associated coiled coil-containing protein kinase (ROCK)-1 has been found to be a new Caspase-3 substrate. ROCK, one of the effectors of the small GTPase Rho, has recently been shown to contribute significantly to myosin light chain (MLC) activation through two pathways: direct phosphorylation of MLC and phosphorylation of MLC phosphatase, leading to its inhibition. The increase in cellular contractility that is necessary for apoptotic membrane blebbing implies sustained augmentation of MLC phosphorylation. ROCK-1 consists of an amino-terminal kinase domain and an inhibitory cysteine/histidine-rich C-terminal domain that is located within a pleckstrin-homology region. They are joined by a variable region that contains the Rho-binding domain. During apoptosis, ROCK-I is cleaved by caspase-3 at a conserved DETD1113/G sequence and its carboxy-terminal inhibitory domain is removed, resulting in deregulated and constitutive kinase activity. The caspase-3-mediated cleavage and activation of ROCK I induces phosphorylation of MLC and membrane blebbing, one of the first events in the execution phase of apoptosis.
Specifications

Specifications

ROCK1 (Cleaved)
Monoclonal
0.5 mg/mL
PBS with 0.05% BSA and 0.05% sodium azide
Q13464, P70335, O77819, Q63644
MGC131603, MGC43611, P160ROCK, PRO0435
Mouse
IgG
100 μg
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Primary
100009220, 19877, 480181, 6093, 81762
Immunocytochemistry, Immunofluorescence, Western Blot
154C1465
Unconjugated
ROCK1
Liquid
ROCK1
Synthetic peptide corresponding to residues G(1114) N L P E S R I E G W L S(1126) of human ROCK-1.
Protein G
RUO
Antibody
Monoclonal
Canine, Human, Mouse, Non-human primate, Rabbit, Rat
Documents
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