ROCK1 (Cleaved Asp1113) Mouse anti-Canine, Human, Mouse, Non-human primate, Rabbit, Rat, Clone: 154C1465, Invitrogen
Mouse Monoclonal Antibody
Manufacturer: Invitrogen MA141031
This mAb recognizes the cleaved C-terminus of ROCK-1. It does not recognize the full-length protein. Since ROCK-1 is cleaved by caspase-3 during apoptosis, this may be used for the detection of apoptosis. A suggested positive control is Jurkat cells treated with camptothecin or anti-Fas antibody to induce apoptosis.The Rho target protein, Rho-associated coiled coil-containing protein kinase (ROCK)-1 has been found to be a new Caspase-3 substrate. ROCK, one of the effectors of the small GTPase Rho, has recently been shown to contribute significantly to myosin light chain (MLC) activation through two pathways: direct phosphorylation of MLC and phosphorylation of MLC phosphatase, leading to its inhibition. The increase in cellular contractility that is necessary for apoptotic membrane blebbing implies sustained augmentation of MLC phosphorylation. ROCK-1 consists of an amino-terminal kinase domain and an inhibitory cysteine/histidine-rich C-terminal domain that is located within a pleckstrin-homology region. They are joined by a variable region that contains the Rho-binding domain. During apoptosis, ROCK-I is cleaved by caspase-3 at a conserved DETD1113/G sequence and its carboxy-terminal inhibitory domain is removed, resulting in deregulated and constitutive kinase activity. The caspase-3-mediated cleavage and activation of ROCK I induces phosphorylation of MLC and membrane blebbing, one of the first events in the execution phase of apoptosis.
|ROCK1 (Cleaved Asp1113)|
|PBS with 0.05% sodium azide|
|Q13464, P70335, O77819, Q63644|
|MGC131603, MGC43611, P160ROCK, PRO0435|
|Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|100009220, 19877, 480181, 6093, 81762|
|Immunocytochemistry, Immunofluorescence, Western Blot|
|Synthetic peptide corresponding to residues G(1114) N L P E S R I E G W L S(1126) of human ROCK-1.|
|Canine, Human, Mouse, Non-human primate, Rabbit, Rat|
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