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CLOCK Rabbit anti-Hamster, Human, Mouse, Rat, Polyclonal, Invitrogen™

Rabbit Polyclonal Antibody

Manufacturer:  Invitrogen PA1520

Catalog No. PA1520


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Description

Description

PA1-520 detects CLOCK from human, mouse, rat and hamster tissues. PA1-520 has been successfully used in Western blot, ICC/IF and immunohistochemistry procedures. By Western blot, this antibody detects an ~100 kDa protein representing CLOCK from rat brain homogenate. Immunohistochemical staining of CLOCK in hamster brain with PA1-520 results in the staining of the superchiasmatic nucleus. PA1-520 immunizing peptide corresponds to amino acid residues 11-30 of mouse CLOCK. This sequence is completely conserved between mouse and human CLOCK. PA1-520 immunizing peptide (Cat. No. PEP-072) is available for use in neutralization and control experiments.

Circadian rhythmicity is a basic property of phylogenetically diverse organisms which range from animals and plants, to fungi. Regulation of endogenous biological clocks is regulated at the genetic level by a protein-mediated, autoregulatory feed-back loop. In mammals, several genes that encode members of the basic helix-loop helix (bHLH) PAS (PER-ARNT-SIM) transcription factor family have been shown to play a significant role in regulating circadian oscillations. Transactivation of CLOCK-induced genes is mediated via an E box enhancer (CACGTG) found upstream of target genes. CLOCK-ARNT3 heterodimers bind to E box regulatory elements and stimulate gene transcription. CLOCK has been shown to transactivate the mammalian homolog of Drosophila per. PER, in concert with the product of the mammalian timeless gene (TIM), negatively regulates its own transcription by blocking the activity of the CLOCK-BMAL1 transactivation complex.
Specifications

Specifications

CLOCK
Polyclonal
Unconjugated
CLOCK
Liquid
CLOCK
Synthetic Peptide: S(11) S I V D R D D S S I F D G L V E E D D(30) C
Antigen affinity chromatography
RUO
Antibody
Polyclonal
Hamster, Human, Mouse, Rat
Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot
1 mg/mL
PBS with 1mg/mL BSA and 0.05% sodium azide
O15516, O08785, Q9WVS9
Circadium Locomotor Output Cycles Kaput
Rabbit
IgG
200 μL
-20° C, Avoid Freeze/Thaw Cycles
Primary
100770092, 12753, 60447, 9575
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