CLOCK Rabbit anti-Mouse, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA1521
Circadian rhythmicity is a basic property of phylogenetically diverse organisms which range from animals and plants, to fungi. Regulation of endogenous biological clocks is regulated at the genetic level by a protein-mediated, autoregulatory feed-back loop. In mammals, several genes that encode members of the basic helix-loop helix (bHLH) PAS (PER-ARNT-SIM) transcription factor family have been shown to play a significant role in regulating circadian oscillations. Transactivation of CLOCK-induced genes is mediated via an E box enhancer (CACGTG) found upstream of target genes. CLOCK-ARNT3 heterodimers bind to E box regulatory elements and stimulate gene transcription. CLOCK has been shown to transactivate the mammalian homolog of Drosophila per. PER, in concert with the product of the mammalian timeless gene (TIM), negatively regulates its own transcription by blocking the activity of the CLOCK-BMAL1 transactivation complex.PA1-521 detects CLOCK from cells transfected with the mouse CLOCK gene.PA1-521 has been sucessfully used in Western blot procedures. By Western blot, this antibody detects an ~100kDa protein representing CLOCK from cells transfected with the mouse CLOCK gene.The PA1-521 immunogen is a synthetic peptide corresponding to residues C S(394) L P E T A A D K S Q D S G S D N (410) of mouse CLOCK. This sequence is completely conserved between mouse and human CLOCK. PA1-521 immunizing peptide (Cat. # PEP-073) is available for use in neutralization and control experiments.
|Synthetic peptide corresponding to residues C S(394) L P E T A A D K S Q D S G S D N (410) of mouse CLOCK.|
|Antigen affinity chromatography|
|PBS with 1mg/mL BSA and 0.05% sodium azide|
|Circadium Locomotor Output Cycles Kaput|
|-20° C, Avoid Freeze/Thaw Cycles|
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