anti-CNPase, Clone: 11-5B, Millipore™ (Chemicon™)

Manufacturer: EMD Millipore MAB326DEL

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Catalog No. 50-175-222


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    Antigen CNPase
    Conjugate Unlabeled
    Cross Reactivity Bovine,Canine,Human,Mouse,Porcine,Rabbit,Rat,Sheep
    Format Semi-purified
    Host Species Mouse
    Immunogen Purified human brain CNPase.
    Isotype IgG1
    Quantity 100μg
    Regulatory Status RUO
    Research Discipline Neuroscience
    Primary or Secondary Primary
    Monoclonal or Polyclonal Monoclonal

    CNPase (2', 3'-cyclic nucleotide 3'-phosphodiesterase [or -phosphohydrolase], EC is present in very high levels in brain and peripheral nerve. This enzyme is found almost exclusively in oligo-dendrocytes and Schwann cells, the cells that form myelin in the central and peripheral nervous system, respectively. Therefore, CNPase has been very useful as a marker for these particular cell types. Developmentally, this enzyme appears early in oligodendrocytes, earlier than most other myelin proteins. It continues to be expressed at high levels in oligodendrocytes and Schwann cells of adult animals. In various diseases, neurological mutants, and in experimental conditions in which myelin is reduced, CNPase levels may be severely reduced. The enzyme itself exists in two forms in most species (three in mouse) with molecular masses of 48 and 46 kD on SDS-PAGE, referred to as CNP2 and CNP1, respectively.Studies continue to elucidate the physiological role of the enzyme. Immunohistochemical localization of CNPase has shown the enzyme to be restricted to oligodendrocytes and Schwann cells. The enzyme appears to be distributed in single and double loose wraps of myelin and not in compact myelin as earlier thought by most investigators. CNPase is located on the inner and outer loops of myelin, paranodally and near the inner surface of the oligodendrocyte membrane. In plaque regions of multiple sclerosis patients, the enzyme is reduced, and when CNS myelin is decreased, CNPase is one of the earlier proteins to be lost from the myelin. In addition, an enzyme that is probably identical to brain CNPase is located in the outer rod segments within the visual system, and this protein is also recognized by the monoclonal antibody 11-5B. In mixed human gliomas, the enzyme levels are reduced, although about 5% of the oligodendrocytes occasionally show normal positive staining.

    Immunocytochemistry, Immunohistochemistry, Immunohistochemistry (Paraffin), Western Blotting

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