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anti-Collagen I alpha 1, Polyclonal, Novus Biologicals™

Rabbit Polyclonal Antibody

Manufacturer:  Novus Biologicals NBP130054

Catalog No. NBP130054

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Collagen I alpha 1 Polyclonal antibody specifically detects Collagen I alpha 1 in Human, Mouse, Rat, Sheep samples. It is validated for Western Blot, Simple Western, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen.


Collagen I alpha 1
1.0 mg/mL
Western Blot 1:1000, Simple Western 1:100, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:100, Immunohistochemistry-Frozen 1:10-1:500
Alpha-1 type I collagen, Collagen 1, Collagen 1 alpha 1, collagen alpha 1 chain type I, collagen alpha-1(I) chain, collagen of skin, tendon and bone, alpha-1 chain, collagen, type I, alpha 1, Collagen1, Collagen-1, OI4, pro-alpha-1 collagen type 1
Immunogen affinity purified
Store at -20C. Avoid freeze-thaw cycles.
Specific for the ~ 140 kDa telopeptide portion of the collagen I alpha 1 polypeptide. The antibody works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90 degrees C in 10 mM sodium citrate (pH 6.0)/ 0.1 % Tween-20). Note that in paraffin sections of formaldehydefixed fibrotic mouse lung tissue, the antibody recognizes mature collagen I that has formed fibrils in the extracellular matrix.
Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot
PBS with No Preservative
Affinity Purified
Synthetic peptide corresponding to amino acid residues within the C-terminal telo peptide portion (aa1193-1218) of the human COL1A1, conjugated to KLH.
140 kDa
0.1 ml
Cytoskeleton Markers, Extracellular Matrix
Human, Mouse, Rat, Sheep
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