Collagen I Rabbit anti-Human, Rat, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA126204
PA1-26204 detects Collagen I from human and rat samples. This antibody exhibits minimal cross reactivity with Collagens 2-6. PA1-26204 has been successfully used in immunoprecipitation, ELISA, Immunohistochemistry (fozen, paraffin) and Western blot procedures. Perform Western blot on non-denatured, non-dissociating protein samples. The PA1-26204 immunogen is Collagen type I extracted and purified from human and bovine placenta by limited pepsin digestion and selective salt precipitation. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.Collagen I is a Type I collagen with a triple helix structure comprised of two alpha-1 chains and one alpha-2 chain. Collagen I is a member of group I collagen (fibril-forming collagen) found in most connective tissues, and is abundant in bone, cornea, dermis and tendon. Mutations in the COL1A2 gene encoding the alpha-2 chain are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in the COL1A2 gene which codes for the alpha-2 chain, however, tend to be less severe than mutations in the COL1A1 gene, reflecting the different role of alpha-2 chains in matrix integrity. Reciprocal translocations between chromosomes 17 and 22, where Collagen Type 1 genes and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified.
|Collagen type I extracted and purified from human and bovine placenta by limited pepsin digestion and selective salt precipitation.|
|Antigen affinity chromatography|
|ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot|
|0.125M sodium borate with 0.075M NaCl, 5mM EDTA and 0.01% sodium azide; pH 8.0|
|Type I Collagen, Collagen 1|
|Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
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