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Collagen I Rabbit anti-Human, Rat, Polyclonal, Invitrogen™

Rabbit Polyclonal Antibody

Manufacturer:  Invitrogen PA126204

Catalog No. PA126204


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Description

Description

PA1-26204 detects Collagen I from human and rat samples. This antibody exhibits minimal cross reactivity with Collagens 2-6. PA1-26204 has been successfully used in immunoprecipitation, ELISA, Immunohistochemistry (fozen, paraffin) and Western blot procedures. Perform Western blot on non-denatured, non-dissociating protein samples. The PA1-26204 immunogen is Collagen type I extracted and purified from human and bovine placenta by limited pepsin digestion and selective salt precipitation. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.

Collagen I is a Type I collagen with a triple helix structure comprised of two alpha-1 chains and one alpha-2 chain. Collagen I is a member of group I collagen (fibril-forming collagen) found in most connective tissues, and is abundant in bone, cornea, dermis and tendon. Mutations in the COL1A2 gene encoding the alpha-2 chain are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in the COL1A2 gene which codes for the alpha-2 chain, however, tend to be less severe than mutations in the COL1A1 gene, reflecting the different role of alpha-2 chains in matrix integrity. Reciprocal translocations between chromosomes 17 and 22, where Collagen Type 1 genes and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified.
Specifications

Specifications

Collagen I
Polyclonal
Unconjugated
COL1A1
Liquid
COL1A1
Collagen type I extracted and purified from human and bovine placenta by limited pepsin digestion and selective salt precipitation.
Antigen affinity chromatography
RUO
Antibody
Polyclonal
Human, Rat
ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
1.1 mg/mL
0.125M sodium borate with 0.075M NaCl, 5mM EDTA and 0.01% sodium azide; pH 8.0
P02452, P02454
Type I Collagen, Collagen 1
Rabbit
IgG
100 μg
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Primary
1277, 29393
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